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KMID : 0364219890320040314
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Korean Journal of Zoology
1989 Volume.32 No. 4 p.314 ~ p.321
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The Ontogenic Analysis of Chick alpha-Fetoprotein
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Park Tae-Kyu
Yoo Jeong-Ah
Jeong Dae-Young
Kang Shin-Sung
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Abstract
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For the preliminary step to investigate the site of AFP synthesis, the ontogenic characteristics and the physiological function of AFP, alpha-fetoprotein was isolated from chick embryo serum through the procedures of CM-Sephadex C-50, hvdroxvapatite and DEAE-Sephadex A-50 chro-matography. The isolated AFP was proved to be pure and its molecular weight was found to be about 60 Kd. With this purified AFP, rabbit anti-chick hor was produced. The serum AFP level in chick embryo has been investagated by using polyacrylamide gel electrophoresis, immunoelec-trophoresis and single radial immunodiffusion from 7 days of incubation until 4 days after hatch-ing. It was found that the AFP level is increased from 0.81 mg/ml at daw-7 embryo to maximum value of 2.46 mg/ml at day-13 embryo, followed by rapid decreases until hatching.
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KEYWORD
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Alpha-Fetoprotein Chick embryo , Ontogeny
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